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KMID : 1100120220290040265
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대한골대사학회지
2022 Volume.29 No. 4 p.265 ~ p.269
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Homogenic Evaluation for Spatial Distribution in Osteoclast Differentiation
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Lim Hyun-Sook
Shin Hong-In
Jeong Dae-Won
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Abstract
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Background: Cells have heterogeneous cellular diversity in size, morphology, cell cycle, metabolism, differentiation degree, and spatial distribution. The shift of specific cells to- wards the desired cells is crucial for maintaining uniform cellular function and can be represented by homogeneity and heterogeneity. Here, we developed a simple and di- rect method for evaluating the homogeneous distribution of desired cells in a constant region.
Methods: We differentiated osteoclast progenitors into bone-resorbing multinu- cleated giant osteoclasts in a 2-dimensional culture plate under 2 conditions. Cells were stained with tartrate-resistant acid phosphatase to assess osteoclast differentiation, im- ages were taken using a microscope and divided into sectors, and the number of osteo- clasts (≥3 nuclei) in each sector was counted. To assess the homogeneity of the spatial distribution of osteoclasts, the standard deviation (SD) was calculated from the mean number of osteoclasts within each sector.
Results: From the 2 groups, a value with a SD close to 0 indicates high spatial homogeneity while a relatively high SD represents low spatial homogeneity.
Conclusions: Our findings suggest that spatial homogeneity can be represented as SD.
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KEYWORD
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Cell differentiation, Homogeneity, Heterogeneity, Osteoclast, Spatial distri-bution
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